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ADInstruments electrocardiogram ecg
Experiment 1: Brain norepinephrine infusion <t>ECG.</t> (a) Mortality during severe hypoglycemia was 16% in aCSF (white bar) and 35% in Nore (black bar) but this was not statistically different. (b) Third‐degree heart block was increased in Nore compared to aCSF. (c) ST elevation was increased in Nore whereas aCSF did not experience ST elevation. (d) Heart rate decreased in both groups during severe hypoglycemia (SH) compared to their respective basals ( p < 0.001). During severe hypoglycemia, Nore had a slight but significantly higher heart rate compared to aCSF ( p < 0.02). (e) QTc prolongation occurred in both groups during severe hypoglycemia compared to basal with no differences between the two groups ( p < 0.001). (f) Representative <t>ECG</t> from aCSF (left) and Nore (right). Basal: Both groups show normal sinus rhythm. T wave increase: Both groups had some rats that experienced this. ST elevation: This did not occur in aCSF. Instead normal sinus rhythm at similar timepoint during severe hypoglycemia is shown. Nore shows ST elevation. Third‐degree heart block: Rats from both groups experienced third‐degree heart block. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–26/group.
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Experiment 1: Brain norepinephrine infusion <t>ECG.</t> (a) Mortality during severe hypoglycemia was 16% in aCSF (white bar) and 35% in Nore (black bar) but this was not statistically different. (b) Third‐degree heart block was increased in Nore compared to aCSF. (c) ST elevation was increased in Nore whereas aCSF did not experience ST elevation. (d) Heart rate decreased in both groups during severe hypoglycemia (SH) compared to their respective basals ( p < 0.001). During severe hypoglycemia, Nore had a slight but significantly higher heart rate compared to aCSF ( p < 0.02). (e) QTc prolongation occurred in both groups during severe hypoglycemia compared to basal with no differences between the two groups ( p < 0.001). (f) Representative <t>ECG</t> from aCSF (left) and Nore (right). Basal: Both groups show normal sinus rhythm. T wave increase: Both groups had some rats that experienced this. ST elevation: This did not occur in aCSF. Instead normal sinus rhythm at similar timepoint during severe hypoglycemia is shown. Nore shows ST elevation. Third‐degree heart block: Rats from both groups experienced third‐degree heart block. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–26/group.
Labchart Pro Software, supplied by POWERLAB INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experiment 1: Brain norepinephrine infusion ECG. (a) Mortality during severe hypoglycemia was 16% in aCSF (white bar) and 35% in Nore (black bar) but this was not statistically different. (b) Third‐degree heart block was increased in Nore compared to aCSF. (c) ST elevation was increased in Nore whereas aCSF did not experience ST elevation. (d) Heart rate decreased in both groups during severe hypoglycemia (SH) compared to their respective basals ( p < 0.001). During severe hypoglycemia, Nore had a slight but significantly higher heart rate compared to aCSF ( p < 0.02). (e) QTc prolongation occurred in both groups during severe hypoglycemia compared to basal with no differences between the two groups ( p < 0.001). (f) Representative ECG from aCSF (left) and Nore (right). Basal: Both groups show normal sinus rhythm. T wave increase: Both groups had some rats that experienced this. ST elevation: This did not occur in aCSF. Instead normal sinus rhythm at similar timepoint during severe hypoglycemia is shown. Nore shows ST elevation. Third‐degree heart block: Rats from both groups experienced third‐degree heart block. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–26/group.

Journal: Physiological Reports

Article Title: Norepinephrine mediates heart block during severe hypoglycemia in male rats

doi: 10.14814/phy2.70010

Figure Lengend Snippet: Experiment 1: Brain norepinephrine infusion ECG. (a) Mortality during severe hypoglycemia was 16% in aCSF (white bar) and 35% in Nore (black bar) but this was not statistically different. (b) Third‐degree heart block was increased in Nore compared to aCSF. (c) ST elevation was increased in Nore whereas aCSF did not experience ST elevation. (d) Heart rate decreased in both groups during severe hypoglycemia (SH) compared to their respective basals ( p < 0.001). During severe hypoglycemia, Nore had a slight but significantly higher heart rate compared to aCSF ( p < 0.02). (e) QTc prolongation occurred in both groups during severe hypoglycemia compared to basal with no differences between the two groups ( p < 0.001). (f) Representative ECG from aCSF (left) and Nore (right). Basal: Both groups show normal sinus rhythm. T wave increase: Both groups had some rats that experienced this. ST elevation: This did not occur in aCSF. Instead normal sinus rhythm at similar timepoint during severe hypoglycemia is shown. Nore shows ST elevation. Third‐degree heart block: Rats from both groups experienced third‐degree heart block. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–26/group.

Article Snippet: An electrocardiogram (ECG) (PowerLab 26 T; LabChart; ADInstruments, Colorado Springs, CO) was recorded throughout the clamp.

Techniques: Blocking Assay, Whisker Assay

Experiment 2: Blockade of norepinephrine with reserpine ECG. (a) Mortality was completely prevented with Reserpine compared to 13.8% in Control (white bar) but this missed significance. (b) Second‐degree heart block was nearly prevented with Reserpine compared to Control. (c) Third‐degree heart block was prevented with Reserpine compared to 24% in Control. (d) T wave increase occurred in Control but was prevented with Reserpine. (e) Heart rate decreased during severe hypoglycemia compared to basal ( p < 0.001) but to a similar extent in both groups. (f) QTc prolongation occurred in both groups compared to basal ( p < 0.001) but to a lesser extent in Reserpine ( p < 0.02). (g) Representative ECG from Control (left) and Reserpine (right) groups. Basal: Rats from both groups were in normal sinus rhythm. Shown under the control group is T wave increase, ST elevation, and third degree heart block. The rats in the Reserpine group did not have T wave increase, ST elevation, or third Degree heart block. Instead, an ECG at a similar timepoint during severe hypoglycemia (i.e., 1, 2, and 3 h) are shown with the rats in normal sinus rhythm. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–29/group.

Journal: Physiological Reports

Article Title: Norepinephrine mediates heart block during severe hypoglycemia in male rats

doi: 10.14814/phy2.70010

Figure Lengend Snippet: Experiment 2: Blockade of norepinephrine with reserpine ECG. (a) Mortality was completely prevented with Reserpine compared to 13.8% in Control (white bar) but this missed significance. (b) Second‐degree heart block was nearly prevented with Reserpine compared to Control. (c) Third‐degree heart block was prevented with Reserpine compared to 24% in Control. (d) T wave increase occurred in Control but was prevented with Reserpine. (e) Heart rate decreased during severe hypoglycemia compared to basal ( p < 0.001) but to a similar extent in both groups. (f) QTc prolongation occurred in both groups compared to basal ( p < 0.001) but to a lesser extent in Reserpine ( p < 0.02). (g) Representative ECG from Control (left) and Reserpine (right) groups. Basal: Rats from both groups were in normal sinus rhythm. Shown under the control group is T wave increase, ST elevation, and third degree heart block. The rats in the Reserpine group did not have T wave increase, ST elevation, or third Degree heart block. Instead, an ECG at a similar timepoint during severe hypoglycemia (i.e., 1, 2, and 3 h) are shown with the rats in normal sinus rhythm. Data are represented as box and whisker plots with median and interquaritle range and min to max values. N = 20–29/group.

Article Snippet: An electrocardiogram (ECG) (PowerLab 26 T; LabChart; ADInstruments, Colorado Springs, CO) was recorded throughout the clamp.

Techniques: Control, Blocking Assay, Whisker Assay